Platelets in Preeclamptic Pregnancies Fail to Exhibit the Decrease in Mitochondrial Oxygen Consumption Rate Seen in Normal Pregnancies

Authors

Joseph Kim, HCA HealthcareFollow
Andrew Malinow, University of Maryland School of Medicine
Rosemary Schuh, University of Marylond School of Medicine

Files

Division

North Texas

Hospital

Medical City Weatherford

Specialty

Internal Medicine

Document Type

Poster

Publication Date

4-28-2023

Keywords

pre-eclampsia, pregnancy-induced hypertension

Disciplines

Female Urogenital Diseases and Pregnancy Complications | Internal Medicine | Obstetrics and Gynecology

Abstract

Cellular oxygen consumption and lactate production rates have been measured in both placental and myometrial cells to study obstetrics-related disease states such as preeclampsia. Platelet metabolic alterations indicate systemic bioenergetic changes that can be useful as disease biomarkers. We tested the hypothesis that platelet mitochondria display functional alterations in preeclampsia. Platelets were harvested from women in the third trimester of either a healthy, non-preeclamptic or preeclamptic pregnancy, and from healthy, non-pregnant women. Using Seahorse respirometry, we analyzed platelets for oxygen consumption (OCR) and extracellular acidification (ECAR) rates, indicators of mitochondrial electron transport and glucose metabolism, respectively. There was a 37% decrease in the maximal respiratory capacity measured in platelets from healthy, non-preeclamptic compared with preeclamptic pregnancy (P

Original Publisher

HCA Healthcare Graduate Medical Education

Recommended Citation

Kim, Joseph; Malinow, Andrew; and Schuh, Rosemary, "Platelets in Preeclamptic Pregnancies Fail to Exhibit the Decrease in Mitochondrial Oxygen Consumption Rate Seen in Normal Pregnancies" (2023). North Texas GME Research Forum 2023. 3.
https://scholarlycommons.hcahealthcare.com/northtexas2023/3